Korystov Y N, Dobrovinskaya O R, Shaposhnikova V V, Eidus L Kh
Laboratory of Cell Radiobiology, Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russian Federation.
FEBS Lett. 1996 Jun 17;388(2-3):238-41. doi: 10.1016/0014-5793(96)00538-8.
The present study demonstrates that DNA fragmentation, nuclear pycnosis and trypan blue staining of irradiated thymocytes is prevented by inhibition of the lipoxygenase pathway of arachidonic acid metabolism and is not affected by cyclooxygenase inhibition. Exposed to irradiation [3H]arachidonic acid-labeled thymocytes release radioactive products to the external medium. The process is blocked by the lipoxygenase inhibitor, nordihydroguaiaretic acid. Thus, it can be concluded that irradiation activates arachidonic acid metabolism and that lipoxygenase metabolites play an important role in thymocyte apoptosis.
本研究表明，抑制花生四烯酸代谢的脂氧合酶途径可防止照射后胸腺细胞的DNA片段化、核固缩和台盼蓝染色，且不受环氧化酶抑制的影响。暴露于辐射下，[3H]花生四烯酸标记的胸腺细胞会向细胞外培养基释放放射性产物。该过程被脂氧合酶抑制剂去甲二氢愈创木酸所阻断。因此，可以得出结论，辐射激活了花生四烯酸代谢，且脂氧合酶代谢产物在胸腺细胞凋亡中起重要作用。
