Patrineli A, Clifford M N, Walker R, Ioannides C
Molecular Toxicology Group, School of Biological Sciences, University of Surrey, Guildford, UK.
Food Chem Toxicol. 1996 Jun;34(6):559-62. doi: 10.1016/0278-6915(96)00015-4.
The mutagenicity of commercially available white grape juice was evaluated in the Ames mutagenicity test. Grape juice elicited a positive mutagenic, response in Salmonella typhimurium strain TA104 and a weaker response in strains TA97, TA98, TA100 and TA1530. The mutagenic response was evident in the absence of an activation system and inclusion of such a system did not influence mutagenicity. The grape juice-mediated mutagenic response was not due to histidine residues in the juice or likely treatment with sulfite. Moreover, freshly prepared grape juice displayed a similar mutagenic response. Three different brands of commercially available white grape juice were investigated in the Ames test; they all provoked a clear positive mutagenic response, but the degree of mutagenicity differed and could not be attributed to differences in the content of solids. It is concluded that grapes contain direct-acting genotoxic component(s).
在艾姆斯致突变试验中评估了市售白葡萄汁的致突变性。葡萄汁在鼠伤寒沙门氏菌TA104菌株中引发了阳性诱变反应，而在TA97、TA98、TA100和TA1530菌株中引发的反应较弱。在没有活化系统的情况下，诱变反应明显，加入这样的系统并不影响致突变性。葡萄汁介导的诱变反应不是由于果汁中的组氨酸残基或可能的亚硫酸盐处理。此外，新鲜制备的葡萄汁也表现出类似的诱变反应。在艾姆斯试验中研究了三种不同品牌的市售白葡萄汁；它们都引发了明显的阳性诱变反应，但致突变程度不同，且不能归因于固体含量的差异。结论是葡萄含有直接作用的遗传毒性成分。
