稻白叶病毒和稗白叶病毒核糖核蛋白的快速小规模纯化
Rapid, small scale purification of rice hoja blanca and Echinochloa hoja blanca tenuivirus ribonucleoprotein.
作者信息
de Miranda J R, Espinoza A M, Hull R
机构信息
CIBCM, University of Costa Rica, San José, Costa Rica.
出版信息
J Virol Methods. 1996 Jan;56(1):109-13. doi: 10.1016/0166-0934(95)01952-9.
Highly purified tenuivirus ribonucleoprotein was obtained from small amounts of leaf tissue by sedimenting the ribonucleoprotein particles from debris-free plant extract into a 30% sucrose cushion, in 1.5-mL microfuge tubes. Using this protocol, significant size differences were discovered in the double-stranded forms of the viral genomic RNAs of rice hoja blanca tenuivirus and a tenuivirus isolated from Echinochloa colonum, a common weed of rice cultivation.
通过在1.5毫升微量离心管中，将无碎片植物提取物中的核糖核蛋白颗粒沉淀到30%的蔗糖垫层上，从少量叶片组织中获得了高度纯化的纤细病毒核糖核蛋白。使用该方案，在水稻白叶病毒和从稻田常见杂草稗草中分离出的一种纤细病毒的病毒基因组RNA双链形式中发现了显著的大小差异。
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