Liu K, Kasper M, Trott K R
Department of Radiation Biology, St Bartholomew's Medical College, London, UK.
Int J Radiat Biol. 1996 Jun;69(6):763-9. doi: 10.1080/095530096145508.
Epidermal stem cells accelerate their repopulation rate during fractionated irradiation. To study the changes in keratinocyte differentiation associated with radiation-induced repopulation, we investigated the expression of a panel of 14 monoclonal or polyclonal antibodies against keratins, involucrin and others, as well as the expression of 15 lectins in the irradiated mouse leg skin. Tissue samples were collected after 1, 2, and 3 weeks of daily irradiation with 3 Gy per fraction. Abnormal morphological appearance of the irradiated epidermis suggested disturbed terminal differentiation. Keratin 16 (K16) was negative in normal epidermis but intense staining was observed in the irradiated epidermis. Involucrin was expressed in the outmost suprabasal layers only in the normal epidermis but extended to the lower layers in the irradiated epidermis. The lectin binding patterns for agglutinins from Soybean, Dolichos biflorus, and Helix pomatia showed differences between the normal and the irradiated epidermis. From these characteristic changes in staining patterns we concluded that accelerated repopulation of the epidermis during fractionated irradiation is associated with a deficiency in terminal squamous differentiation.
表皮干细胞在分次照射期间会加快其再增殖速率。为了研究与辐射诱导的再增殖相关的角质形成细胞分化变化，我们调查了一组针对角蛋白、内披蛋白等的14种单克隆或多克隆抗体的表达，以及15种凝集素在受照射小鼠腿部皮肤中的表达。每天以3 Gy的剂量分次照射，在照射1、2和3周后收集组织样本。受照射表皮的异常形态外观提示终末分化受到干扰。角蛋白16（K16）在正常表皮中呈阴性，但在受照射表皮中观察到强烈染色。内披蛋白仅在正常表皮的最上层基底上层表达，但在受照射表皮中延伸至下层。来自大豆、双花扁豆和苹果蜗牛的凝集素的凝集素结合模式在正常表皮和受照射表皮之间存在差异。从这些染色模式的特征性变化中我们得出结论，分次照射期间表皮的加速再增殖与终末鳞状分化缺陷有关。
