Wilsson A, Lundqvist H, Gustafsson M, Stendahl O
Department of Medical Microbiology, Linköping University, Sweden.
J Leukoc Biol. 1996 Jun;59(6):902-7. doi: 10.1002/jlb.59.6.902.
The mobilization of intracellular calcium plays an important role in regulating neutrophil activation. With this in mind we investigated the effect of intra- and extracellular calcium on the ability of human neutrophils to kill complement-opsonized Staphylococcus aureus. We found that a rise in intracellular calcium is necessary for efficient killing of phagocytosed S. aureus. In the presence of extracellular calcium, killing of ingested bacteria in calcium-buffered neutrophils compared with normal cells was slightly reduced. Calcium buffering had no effect on phagocytic uptake by the neutrophils, but did decrease the generation of toxic oxygen metabolites, measured as chemiluminescence (CL). In nondepleted and calcium-depleted cells, removal of extracellular calcium did not affect ingestion but did cause a marked decrease in the ability to kill the bacteria. In parallel, the CL response was substantially reduced or completely blocked. These data show that calcium is not a prerequisite for phagocytosis of S. aureus by human neutrophils, but does play a vital role in the post-ingestion killing of the bacteria by regulating the generation of toxic oxygen metabolites.
细胞内钙的动员在调节中性粒细胞活化中起重要作用。基于此，我们研究了细胞内和细胞外钙对人中性粒细胞杀伤补体调理的金黄色葡萄球菌能力的影响。我们发现细胞内钙升高对于有效杀伤吞噬的金黄色葡萄球菌是必要的。在存在细胞外钙的情况下，与正常细胞相比，钙缓冲的中性粒细胞中摄入细菌的杀伤略有降低。钙缓冲对中性粒细胞的吞噬摄取没有影响，但确实减少了以化学发光（CL）测量的有毒氧代谢产物的产生。在未耗尽和钙耗尽的细胞中，去除细胞外钙不影响摄取，但确实导致杀伤细菌的能力显著下降。同时，CL反应大幅降低或完全受阻。这些数据表明，钙不是人中性粒细胞吞噬金黄色葡萄球菌的先决条件，但在摄入后通过调节有毒氧代谢产物的产生对细菌的杀伤中起着至关重要的作用。
