通过流式细胞术检测感染吉氏巴贝斯虫的犬红细胞。
Detection of canine erythrocytes infected with Babesia gibsoni by flow cytometry.
作者信息
Fukata T, Ohnishi T, Okuda S, Sasai K, Baba E, Arakawa A
机构信息
Department of Veterinary Medicine, College of Agriculture, Osaka Prefecture University, Japan.
出版信息
J Parasitol. 1996 Aug;82(4):641-2.
PMID:8691376
Abstract
Peripheral blood samples from dogs infected with Babesia gibsoni were analyzed by flow cytometry for parasitized erythrocytes after staining with hydroethidine. Cells identified as positive by flow cytometry were erythrocytes infected with B. gibsoni. Analysis of 26 samples by flow cytometry for % parasitemia revealed a correlation coefficient of 0.97 in comparison to the conventional method of light microscopy.
摘要
用氢乙锭染色后，通过流式细胞术对感染吉氏巴贝斯虫的犬外周血样本中的寄生红细胞进行分析。通过流式细胞术鉴定为阳性的细胞是感染了吉氏巴贝斯虫的红细胞。通过流式细胞术分析26个样本的疟原虫血症百分比，与传统光学显微镜方法相比，相关系数为0.97。
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