Fan S R, Ho I C, Yeoh F L, Lin C J, Lee T C
Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, ROC.
Mutat Res. 1996 Jul 5;368(3-4):165-9. doi: 10.1016/s0165-1218(96)90058-0.
Arsenic, widely distributed throughout our environment, is a well-established human carcinogen. We report here that squalene, a natural fish oil, is a potential agent in the reduction of sodium arsenite-induced sister chromatid exchange (SCE) and micronuclei in Chinese hamster ovary (CHO-K1) cells. Squalene dose-dependently inhibited sodium arsenite-induced SCE. At the highest concentration (160 microM), squalene reduced the SCE frequency from 8.85 to 6.47 SCEs per cell which is very close to the background level (5.82 SCEs per cell). Sodium arsenite dose-dependently induces micronuclei in CHO-K1 cells, and squalene at 80 microM significantly inhibits arsenite-induced micronuclei. However, squalene did not eliminate the killing effects of arsenite on the cells and only slightly decreased intracellular accumulation of arsenic.
砷广泛分布于我们的环境中，是一种公认的人类致癌物。我们在此报告，角鲨烯这种天然鱼油，是一种有可能减少亚砷酸钠诱导的中国仓鼠卵巢（CHO-K1）细胞姐妹染色单体交换（SCE）和微核的物质。角鲨烯剂量依赖性地抑制亚砷酸钠诱导的SCE。在最高浓度（160微摩尔）时，角鲨烯将SCE频率从每细胞8.85次SCE降低至6.47次SCE，这非常接近背景水平（每细胞5.82次SCE）。亚砷酸钠剂量依赖性地诱导CHO-K1细胞产生微核，80微摩尔的角鲨烯显著抑制亚砷酸盐诱导的微核。然而，角鲨烯并未消除亚砷酸盐对细胞的杀伤作用，只是略微降低了细胞内砷的积累。
