启动子处必需的激活因子 - 聚合酶添加顺序。
Obligatory activator-polymerase addition order at promoters.
作者信息
Zhang X, Schleif R
机构信息
Biology Department, Johns Hopkins University, Baltimore, MD 21218, USA.
出版信息
Nucleic Acids Res. 1996 Jul 1;24(13):2575-7. doi: 10.1093/nar/24.13.2575.
Abstract
The kinetics of open complex formation were measured by migration retardation assay and DNase I footprinting at the activator-dependent promoters ara P1, lac P1 and gal P1. In each case, the rate of open complex formation was significantly faster if the activator, AraC for ara and CAP for lac and gal, had been added before RNA polymerase. The results indicate that complexes of transcriptional activators, RNA polymerase and promoter can exist in two states, one which can form open complexes rapidly and one which cannot.
摘要
通过迁移阻滞分析和DNase I足迹法，在依赖激活剂的ara P1、lac P1和gal P1启动子处测定开放复合物形成的动力学。在每种情况下，如果在RNA聚合酶之前加入激活剂（ara的AraC以及lac和gal的CAP），开放复合物形成的速率会显著加快。结果表明，转录激活剂、RNA聚合酶和启动子的复合物可以存在两种状态，一种能够快速形成开放复合物，另一种则不能。
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本文引用的文献
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AraC protein can activate transcription from only one position and when pointed in only one direction.阿糖胞苷蛋白只能从一个位置并仅在一个方向上激活转录。
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Regulation of the Escherichia coli L-arabinose operon studied by gel electrophoresis DNA binding assay.通过凝胶电泳DNA结合试验研究大肠杆菌L-阿拉伯糖操纵子的调控
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