Francia M V, García Lobo J M
Departamento de Biología Molecular, Facultad de Medicina Universidad de Cantabria, Santander, Spain.
Plasmid. 1996 Jan;35(1):31-6. doi: 10.1006/plas.1996.0003.
A 580-bp-long stem-loop DNA (sl-DNA) molecule was produced by a recombinant plasmid containing complete int and aadA genes from the transposon Tn21 cloned in the vector pUC18. A 50-bp-long inverted repeat found near the int gene stop codon could be involved in the generation of the sl-DNA molecule. cloning of the dhfrII gene between the plasmid replication origin and this IR produced an extended sl-DNA including the dhfrII gene. Tn21 recombination hot spot 2 was also able to direct the production of sl-DNA.
