Langer T, Möstl E, Chizzola R, Gutleb R
Institut für Botanik und Lebensmittelkunde, Veterinärmedizinische Universität Wien, Austria.
Planta Med. 1996 Jun;62(3):267-71. doi: 10.1055/s-2006-957875.
We developed an immunoassay with antibodies against retrorsine to detect the closely related senecionine, the main alkaloid in various Asteraceae. Sensitivity is about 23 pg (68 fmol) for senecionine. Cross reactivity of monocrotalin, retrorsine N-oxide, senkirkine (< 0.1%), and seneciphylline (3.6-34.5%) was resolved. The alkaloid content in leaves of Petasites hybridus (Asteraceae) was determined to be 3.86 ppm, calculated as senecionine, which was far less than in rhizomes (104.8 ppm). Additionally, we proved specificity by taking immunograms from different parts of Petasites hybridus. The test is a suitable tool for assessing the toxic potential of medicinal plants containing senecionine.
我们开发了一种针对倒千里光碱的免疫分析法，用于检测与之密切相关的千里光宁碱，它是各种菊科植物中的主要生物碱。千里光宁碱的检测灵敏度约为23皮克（68飞摩尔）。解决了农吉利碱、倒千里光碱N-氧化物、山梗菜碱（<0.1%）和千里光菲灵碱（3.6 - 34.5%）的交叉反应性问题。以千里光宁碱计算，蜂斗菜（菊科）叶片中的生物碱含量测定为3.86 ppm，远低于根茎中的含量（104.8 ppm）。此外，我们通过对蜂斗菜不同部位进行免疫图谱分析证明了其特异性。该检测方法是评估含有千里光宁碱的药用植物潜在毒性的合适工具。
