Juris P, Tóth F, Lauková A, Plachý P, Dubinský P, Sokol J
Parasitological Institute of the Slovak Academy of Sciences, Kosice, Slovak Republic.
Vet Med (Praha). 1996 May;41(5):149-53.
The effect of methanogenesis on the survival of model bacterial strains (Escherichia coli EC 5, Staphylococcus aureus SA 11, Enterococcus faecium CCM 4231) and non-embryonated helminth eggs (Ascaris suum) was investigated in pig slurry. Two pilot-plant experiments were carried out in two anaerobic digesters (800 and 1,000 litre) in a mesophilic thermal range (35-37 degrees C). The mean hydraulic retention time of the digesters was 20 days. The methanogenesis process was monitored by determining the following chemical parameters: pH, N-NH3, total dry matter (kg/day), organic matter (kg/day) production of methane by supplied and degraded organic matter (m3/kg). The results obtained allow us to state that the anaerobic stabilization of pig slurry in the mesophilic temperature range resulted in total devitalization of model bacterial strains E. coli EC 5 and Ent. faecium CCM 4231. St. aureus SA 11 cells, exposed to the above mentioned conditions, were also reduced in their number from 10.04 to 3.27 and from 8.69 to 2.77 log cfu/ml. It is assumed that the longer retention time of excrements in the digester could also result in total devitalization of St. aureus SA 11 cells. From the microbiological point of view, the above mentioned facts indicate a sufficient hygienization effect of the anaerobic fermentation on the contaminated pig excrements. The survival of A. suum eggs was little affected by the 20-day anaerobic mesophilic digestion of pig slurry. Only 17 or 18% (F1, F2) of the non-embryonated A. suum eggs were damaged after the 20-day exposure.
研究了产甲烷作用对猪粪中模型细菌菌株（大肠杆菌EC 5、金黄色葡萄球菌SA 11、粪肠球菌CCM 4231）和未受精蠕虫卵（猪蛔虫）存活的影响。在两个中温范围（35 - 37摄氏度）的厌氧消化器（800升和1000升）中进行了两项中试实验。消化器的平均水力停留时间为20天。通过测定以下化学参数来监测产甲烷过程：pH值、N - NH₃、总干物质（千克/天）、有机物质（千克/天）、供应和降解有机物质产生的甲烷量（立方米/千克）。所获得的结果使我们能够指出，猪粪在中温温度范围内的厌氧稳定化导致模型细菌菌株大肠杆菌EC 5和粪肠球菌CCM 4231完全失活。暴露于上述条件下的金黄色葡萄球菌SA 11细胞数量也从10.04降至3.27 log cfu/ml，从8.69降至2.77 log cfu/ml。据推测，粪便在消化器中停留时间延长也可能导致金黄色葡萄球菌SA 11细胞完全失活。从微生物学角度来看，上述事实表明厌氧发酵对受污染猪粪具有足够的卫生化效果。猪蛔虫卵的存活受猪粪20天中温厌氧消化的影响较小。暴露20天后，只有17%或18%（F1、F2）的未受精猪蛔虫卵受损。
