Silver H M, Struminsky J
Division of Maternal-Fetal Medicine, Women and Infants' Hospital of Rhode Island, Providence, USA.
Am J Obstet Gynecol. 1996 Jul;175(1):155-7. doi: 10.1016/s0002-9378(96)70266-1.
Our purpose was to compare the yield of positive group B Streptococcus cultures with standard medium for transport of culture swabs compared with use of selective medium during transport.
Cultures of introitus, perineum, and rectum were obtained on prenatal patients; one was placed in standard transport medium, and the other directly in selective growth medium. Swabs in standard transport medium were plated for routine culture and then transferred to selective growth medium, Todd-Hewitt broth, in the laboratory.
A total of 307 of 1222 (25.1%) patients had a positive result by any method. With direct inoculation into selective growth medium at the time of sampling, 4.6% of positive cultures were missed. With delayed inoculation into selective growth medium, 16.3% were missed (p < 0.001). Without use of selective media (routine culture), 31.9% were missed (p < 0.001).
Use of standard transport medium with subsequent transfer into selective growth medium results in a significantly decreased yield of positive group B Streptococcus cultures.
我们的目的是比较用于培养拭子运输的标准培养基与运输过程中使用选择性培养基时B族链球菌培养阳性率。
对产前患者的阴道、会阴和直肠进行培养；一个样本置于标准运输培养基中，另一个直接置于选择性生长培养基中。置于标准运输培养基中的拭子先进行常规培养，然后在实验室转移至选择性生长培养基（托德-休伊特肉汤）中。
1222例患者中共有307例（25.1%）通过任何方法检测结果为阳性。采样时直接接种到选择性生长培养基中，漏检了4.6%的阳性培养物。延迟接种到选择性生长培养基中，漏检了16.3%（p<0.001）。不使用选择性培养基（常规培养）时，漏检了31.9%（p<0.001）。
使用标准运输培养基随后转移至选择性生长培养基会导致B族链球菌培养阳性率显著降低。
