Wu G, Zhang F, Salley R K, Robinson M C, Chien S
Department of Surgery, University of Kentucky Medical Center, Lexington, USA.
Ann Thorac Surg. 1996 Aug;62(2):356-62.
Testing lung function after preservation is difficult because a suitable model is still lacking; thus, the effectiveness of different solutions for lung preservation has not been confirmed. This study tested the effectiveness of Euro-Collins solution alone for hypothermic preservation of rat lungs.
A living rat perfusion model was used, which allowed more than 5 hours of continuous perfusion for isolated lung function studies. Group 1 lungs (control, n = 8) were tested without preservation. In groups 2 through 6 (n = 8 lungs each), the lungs were flushed with 4 degrees C Euro-Collins solution and preserved for 4, 6, 8, 12, and 24 hours, respectively. Lung function studies were carried out after preservation.
In groups 1 and 2, pulmonary arterial blood flow and pulmonary venous oxygen tension were higher and pulmonary resistance was lower than in the other groups. Airway pressure and resistance were lowest in group 1. Lungs in groups 5 and 6 demonstrated the worst function, but the lung tissue wet to dry ratio was higher only in group 6.
At 4 degrees C, Euro-Collins solution can effectively preserve rat lungs for 4 hours. Six to 8 hours of preservation resulted in depressed lung function. More than 12 hours of preservation resulted in uniformly deficient lung function, rendering the lungs unsuitable for transplantation.
由于仍缺乏合适的模型，保存后测试肺功能很困难；因此，不同肺保存溶液的有效性尚未得到证实。本研究测试了单用欧洲柯林斯液对大鼠肺进行低温保存的有效性。
采用活体大鼠灌注模型，该模型可使离体肺功能研究连续灌注超过5小时。第1组肺（对照组，n = 8）未进行保存直接测试。第2至6组（每组n = 8个肺）的肺用4℃欧洲柯林斯液冲洗，分别保存4、6、8、12和24小时。保存后进行肺功能研究。
第1组和第2组的肺动脉血流量和肺静脉氧分压高于其他组，肺阻力低于其他组。第1组的气道压力和阻力最低。第5组和第6组的肺功能最差，但仅第6组的肺组织湿干比更高。
在4℃时，欧洲柯林斯液可有效保存大鼠肺4小时。保存6至8小时会导致肺功能下降。保存超过12小时会导致肺功能普遍不足，使肺不适合移植。
