Husain D, Miller J W, Michaud N, Connolly E, Flotte T J, Gragoudas E S
Laser Research Laboratory, Massachusetts Eye and Ear Infirmary, Boston, USA.
Arch Ophthalmol. 1996 Aug;114(8):978-85. doi: 10.1001/archopht.1996.01100140186012.
To compare the effectiveness of photodynamic therapy to close experimental choroidal neovascularization using an intravenous infusion of liposomal benzoporphyrin derivative (verteporfin) with previous work using a rapid intravenous injection, before initiating clinical trials.
Choroidal neovascularization was induced in cynomolgus monkey eyes using argon laser. Liposomal benzoporphyrin derivative was delivered by an intravenous infusion pump for 10 or 32 minutes at a dose of 0.375 mg/kg. Irradiation was performed with 689- or 692-nm laser light (600-mW/cm2 irradiance and 150-J/cm2 fluence) in 7 normal eyes and 11 eyes with choroidal neovascularization between 30 and 105 minutes after the start of dye infusion. Findings were documented by fundus photography, fluorescein angiography, and light and electron microscopy.
Irradiation within 32 to 50 minutes of the start of the fast (10 minutes) or slow (32 minutes) dye infusion resulted in closure of choroidal neovascularization. In normal eyes, this technique caused choriocapillaris closure and retinal pigment epithelium damage with minimal damage to surrounding tissues.
Photodynamic therapy using intravenous infusion of liposomal benzoporphyrin derivative selectively closed experimental choroidal neovascularization. This may be a suitable modality for clinical use.
在开展临床试验之前，比较静脉输注脂质体苯卟啉衍生物（维替泊芬）进行光动力疗法以封闭实验性脉络膜新生血管的效果与之前快速静脉注射的研究结果。
使用氩激光诱导食蟹猴眼出现脉络膜新生血管。通过静脉输液泵以0.375mg/kg的剂量输注脂质体苯卟啉衍生物10或32分钟。在开始输注染料后30至105分钟内，用689或692nm激光（辐照度600mW/cm²，能量密度150J/cm²）对7只正常眼和11只患有脉络膜新生血管的眼睛进行照射。通过眼底照相、荧光素血管造影以及光镜和电镜记录结果。
在快速（10分钟）或缓慢（32分钟）输注染料开始后的32至50分钟内进行照射可导致脉络膜新生血管闭合。在正常眼中，该技术导致脉络膜毛细血管闭合和视网膜色素上皮损伤，而对周围组织的损伤最小。
静脉输注脂质体苯卟啉衍生物进行光动力疗法可选择性地封闭实验性脉络膜新生血管。这可能是一种适合临床应用的方法。
