Sjoblad R D, Bollag J M
Appl Environ Microbiol. 1977 Apr;33(4):906-10. doi: 10.1128/aem.33.4.906-910.1977.
The soil fungus Rhizoctonia praticola produced an enzyme that accumulated in the growth medium and caused the polymerization of phenolic and naphtholic intermediates of various pesticides. The dialyzed crude enzyme was purified by ion-exhange column chromatography with diethylaminoethyl-cellulose, followed by gel filtration with Sephadex G-200. The enzyme, a phenol oxidase, was capable of polymerizing 2-chlorophenol, 4-chlorophenol, 2,4-dichlorophenol, and 4-bromo-2-chlorophenol. 1-Naphthol, 2-naphthol, and some of their derivatives formed oligomers or polymers when incubated with the enzyme, but 4-nitrophenol and 2,4-dinitriphenol were not oxidized. Chlorinated and brominated anilines, which are derivatives of herbicides, were not altered by the phenol oxidase from R. praticola, but 4-methoxyaniline was transformed by the enzyme to 2-amino-5-p-anisidinobenzoquinone-di-p-methoxyphenylimine. The formation of polymeric products was determined by mass spectrometric analysis.
土壤真菌立枯丝核菌产生一种酶，该酶在生长培养基中积累，并导致各种农药的酚类和萘酚类中间体发生聚合。经透析的粗酶先用二乙氨基乙基纤维素通过离子交换柱色谱法进行纯化，然后用葡聚糖G - 200进行凝胶过滤。该酶是一种酚氧化酶，能够使2 - 氯酚、4 - 氯酚、2,4 - 二氯酚和4 - 溴 - 2 - 氯酚发生聚合。1 - 萘酚、2 - 萘酚及其一些衍生物在与该酶一起孵育时会形成低聚物或聚合物，但4 - 硝基酚和2,4 - 二硝基酚不会被氧化。作为除草剂衍生物的氯化和溴化苯胺不会被立枯丝核菌的酚氧化酶改变，但4 - 甲氧基苯胺会被该酶转化为2 - 氨基 - 5 - 对甲氧基苯胺基苯醌 - 二 - 对甲氧基苯基亚胺。通过质谱分析确定聚合物产物的形成。
