Huber C, Christophers E
Arch Dermatol Res (1975). 1977 Apr 27;258(2):169-73. doi: 10.1007/BF00561622.
The microscopical changes of plantar Str. corneum after immersion in alkaline were investigated. For this purpose a test system was used in which unexcised skin of guinea pig hind feet was exposed and subsequently analysed by the FITC-staining method. The effect of KOH on the Str. corneum consisted in an immediate swelling of the outer cell layers which was followed by a further increase in cell volume. Cells became separated layer by layer after maximal swelling. There is a high and constant rate of cell loss, when treated superficial cells are repeatedly removed as compared to permanent immersion. Thus, the buffering capacity of the treated outer cell layers has a rate limiting effect upon alkaline-induced cell loss in the Str. corneum.
研究了碱液浸泡后足底角质层的微观变化。为此，使用了一种测试系统，其中豚鼠后足未切除的皮肤被暴露，随后通过FITC染色法进行分析。氢氧化钾对角质层的作用表现为外层细胞立即肿胀，随后细胞体积进一步增加。最大肿胀后，细胞逐层分离。与持续浸泡相比，当反复去除处理过的表层细胞时，细胞损失率高且恒定。因此，处理过的外层细胞层的缓冲能力对角质层中碱诱导的细胞损失具有限速作用。
