长距离聚合酶链反应在λ EMBL3噬菌体载体上克隆DNA片段的限制性酶切位点图谱绘制中的应用。
Application of long-distance PCR to restriction site mapping of a cloned DNA fragment on the lambda EMBL3 phage vector.
作者信息
Machida M, Manabe M, Yasukawa M, Jigami Y
机构信息
Department of Molecular Biology, National Institute of Bioscience and Human-Technology, Ibaraki, Japan.
出版信息
Biosci Biotechnol Biochem. 1996 Jun;60(6):1011-3. doi: 10.1271/bbb.60.1011.
Long-distance PCR was applied to rapidly map the restriction sites of long inserts cloned on lambda EMBL3 phage vector. The restriction sites of 9 of 15 enzymes were completely assigned in a model experiment within 14 h, including 8h for the PCR amplification. This method was found particularly useful for genomic DNA cloning when the partial sequence of the corresponding cDNA is known.
长距离聚合酶链反应（Long-distance PCR）被用于快速定位克隆在λEMBL3噬菌体载体上的长插入片段的限制性酶切位点。在一个模型实验中，15种酶中的9种酶的限制性酶切位点在14小时内被完全确定，其中包括8小时的PCR扩增时间。当相应cDNA的部分序列已知时，该方法被发现对基因组DNA克隆特别有用。
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