Dai Y, Chen H, Wang N
Department of Pathology, Secondary Military Medical University, Shanghai.
Zhonghua Yi Xue Za Zhi. 1995 Nov;75(11):666-9, 709.
Immunoactive cell group markers and glycoproteins of extracellular matrix and basement membrane (fibronectin, laminin, type IV collage) in 156 human hepatocellular carcinomas and their surrounding tissues were localized with immunoperoxidase technique. T lymphocytes were the major immunoactive cells in the regions of local invasion in liver cancer. The distributing patterns of the glycoproteins were similar to the results of previous reports, mainly localized in the surrounding cancer cells, interstitium and vessel walls or sinusoids in tumor, and tumor capsule. The patients, whose hepatocarcinomas showed stronger cell immunoactivity (T and help T cells were in the majority) and higher expression of glycoproteins of extracellular matrix and basement membrane had a longer tumor-free survival (63.2 to 12.8 months) and lower recurrences (P < 0.01). Of the 156 patients 92.9%, 82.6%, 65.4%, 44.2% and 30.8% and 1, 2, 3, 4 and 5 postoperative tumor-free years respectively with a total currence rate of 53.2% (83 patients). In the 83 recurrences, 65 were intrahepatic subclinical, which were promptly reresected 78.3%, recurrence related factors included tumor number and size, capsule infiltration, and portal veins involvement. Being different from those with single node, capsulated hepatocellular carcinomas and multiple/daughter ones had invisible tumor cells disseminated to the remnant liver. Those without capsule infiltration had a low recurrence rate, which agrees to the hypothesis that capsules can bar the dissemination of tumor cells. Additional results show that postoperative tumor recurrence is mainly pertinent to histopathological characteristics of the primary focus. According to the phenomenon that nearly 63.1% of recurrent liver carcinomas are located at the ipsilateral segment of the primary ones, we emphasize that the occurrence and recurrence of liver carcinoma are mainly unicentral.
采用免疫过氧化物酶技术对156例人肝细胞癌及其周围组织中的免疫活性细胞群标志物以及细胞外基质和基底膜的糖蛋白（纤连蛋白、层粘连蛋白、IV型胶原）进行定位。T淋巴细胞是肝癌局部浸润区域的主要免疫活性细胞。糖蛋白的分布模式与先前报道的结果相似，主要定位于肿瘤周围的癌细胞、间质、肿瘤内的血管壁或血窦以及肿瘤包膜。肝细胞癌细胞免疫活性较强（以T细胞和辅助性T细胞为主）且细胞外基质和基底膜糖蛋白表达较高的患者，无瘤生存期较长（63.2至12.8个月）且复发率较低（P<0.01）。156例患者术后分别有92.9%、82.6%、65.4%、44.2%和30.8%的患者无瘤生存1、2、3、4和5年，总复发率为53.2%（83例）。在83例复发患者中，65例为肝内亚临床复发，其中78.
