Dhib-Jalbut S, Jiang H, Xia Q, Blatt L, Johnson K P, Hilt D
Department of Neurology, University of Maryland at Baltimore 21201, USA.
J Interferon Cytokine Res. 1996 Mar;16(3):195-200. doi: 10.1089/jir.1996.16.195.
Interferon-consensus 1 (IFN-Con 1) is a novel synthetic protein generated from codons for the most frequent amino acids in different type 1 IFNs. Compared with natural IFNs, IFN-Con 1 has been shown to have higher specific activity and antiproliferative activity and a higher ability to induce natural killer cells. In this study, the effects of IFN-Con 1 were compared with those of IFN-beta 1b and IFN-alpha 2a on HLA expression and lymphoproliferation. Human umbilical vein endothelial cells (HUVEC) express HLA class I but not class II molecules; however, both class I and class II molecules can be upregulated by IFN-gamma. IFN-Con-1 shared with IFN-beta 1b and IFN-alpha 2a the capacity to enhance HLA class I expression on HUVEC, alone and in combination with IFN-gamma. Although IFN-Con 1 had no effect on the basal expression of HLA class II molecules, it inhibited the IFN-gamma-induced class II expression on the HUVEC in a dose-dependent fashion. When this effect was compared among the three IFNs on mass basis, IFN-Con 1 activity was intermediate between that of IFN-beta 1b and IFN-alpha 2a. IFN-Con 1 also demonstrated an inhibitory effect on mitogen-driven lymphoproliferation similar to that of IFN-alpha 2a and exceeded that of IFN-beta 1b. The results indicate that IFN-Con 1 has immunomodulatory effects similar to those of IFN-beta 1b and IFN-alpha 2a, which could be relevant to the treatment of autoimmune and virus-mediated diseases.
干扰素共识1（IFN-Con 1）是一种新型合成蛋白，由不同1型干扰素中最常见氨基酸的密码子生成。与天然干扰素相比，IFN-Con 1已显示出具有更高的比活性和抗增殖活性，以及更高的诱导自然杀伤细胞的能力。在本研究中，比较了IFN-Con 1与IFN-β1b和IFN-α2a对HLA表达和淋巴细胞增殖的影响。人脐静脉内皮细胞（HUVEC）表达HLA I类分子但不表达II类分子；然而，I类和II类分子均可被IFN-γ上调。IFN-Con-1与IFN-β1b和IFN-α2a一样，具有单独或与IFN-γ联合增强HUVEC上HLA I类分子表达的能力。尽管IFN-Con 1对HLA II类分子的基础表达没有影响，但它以剂量依赖的方式抑制IFN-γ诱导的HUVEC上II类分子的表达。当在质量基础上比较这三种干扰素的这种作用时，IFN-Con 1的活性介于IFN-β1b和IFN-α2a之间。IFN-Con 1对丝裂原驱动的淋巴细胞增殖也表现出抑制作用，类似于IFN-α2a，且超过IFN-β1b。结果表明，IFN-Con 1具有与IFN-β1b和IFN-α2a相似的免疫调节作用，这可能与自身免疫性疾病和病毒介导疾病的治疗有关。
