Kolosov M, Kolosova I, leu R W
Oklahoma Medical Research Foundation, Noble Center for Biomedical Research, Oklahoma City 73104-5046, USA.
J Interferon Cytokine Res. 1996 Mar;16(3):245-9. doi: 10.1089/jir.1996.16.245.
Both exogenous interferon-gamma (IFN-gamma) and interferon-alpha/beta (IFN-alpha/beta) stimulate C1q mRNA synthesis in murine macrophages. Previous studies suggested that IFN-gamma induced endogenous synthesis of IFN-alpha/beta by murine macrophages. In the present study, we determined the indirect effect of IFN-gamma on macrophage synthesis of C1q mRNA mediated by endogenous IFN-alpha/beta production. Upregulation of macrophage synthesis of C1q mRNA by both IFN-gamma and IFN-alpha/beta was reversed by neutralizing antibody to IFN-alpha/beta but not by nonimmune control serum. IFN-gamma was confirmed by ELISA to stimulate a six- to eightfold increase in macrophage secretion of IFN-alpha/beta compared with untreated control cells. We tentatively conclude that IFN-gamma stimulates macrophage synthesis and secretion of IFN-alpha/beta, which in turn promotes C1q mRNA synthesis in an autocrine or paracrine manner. Thus, exogenous IFN-gamma, derived from activated T lymphocytes, may act primarily on the macrophage to stimulate the endogenous synthesis of IFN-alpha/beta for autocrine modulation of a variety of biologic functions, including C1q synthesis.
外源性γ干扰素（IFN-γ）和α/β干扰素（IFN-α/β）均可刺激小鼠巨噬细胞中C1q mRNA的合成。先前的研究表明，IFN-γ可诱导小鼠巨噬细胞内源性合成IFN-α/β。在本研究中，我们确定了IFN-γ通过内源性IFN-α/β产生对巨噬细胞合成C1q mRNA的间接作用。IFN-γ和IFN-α/β对巨噬细胞C1q mRNA合成的上调作用，可被IFN-α/β的中和抗体逆转，但不能被非免疫对照血清逆转。通过酶联免疫吸附测定（ELISA）证实，与未处理的对照细胞相比，IFN-γ可刺激巨噬细胞分泌的IFN-α/β增加6至8倍。我们初步得出结论，IFN-γ刺激巨噬细胞合成和分泌IFN-α/β，进而以自分泌或旁分泌方式促进C1q mRNA的合成。因此，源自活化T淋巴细胞的外源性IFN-γ可能主要作用于巨噬细胞，刺激IFN-α/β的内源性合成，从而自分泌调节包括C1q合成在内的多种生物学功能。
