Kristensen G B, Holm R, Abeler V M, Tropé C G
Department of Gynecologic Oncology, The Norwegian Radium Hospital, Oslo, Norway.
Cancer. 1996 Aug 1;78(3):433-40. doi: 10.1002/(SICI)1097-0142(19960801)78:3<433::AID-CNCR9>3.0.CO;2-K.
This study evaluated the prognostic significance of immunohistochemical staining for cathepsin D, epidermal growth factor receptor (EGFR), and c-erbB-2 in patients with early cervical squamous cell carcinoma.
This retrospective analysis comprised 132 patients, all subjected to radical hysterectomy with bilateral pelvic lymphadenectomy for International Federation of Gynecology and Obstetrics (FIGO) Stage IB cervical squamous cell carcinoma. Immunohistochemical staining was correlated with various histopathologic and morphologic characteristics (i.e., tumor size, grade of differentiation, vessel invasion, invasion into parametria, and lymph node metastasis) and relapse free survival.
Positive staining for cathepsin D was observed in 47% of tumors, more frequent in tumors giving rise to lymph node metastases. The relapse free survival was lower for patients with cathepsin D positive tumors. Overexpression of EGFR was observed in 25.8% of the tumors. There was no correlation with any of the histopathologic variables investigated. Relapse free survival was lower for patients with tumors overexpressing EGFR. Immunohistochemical staining for c-erbB-2 was observed in 12.1% of tumors with no correlation with relapse free survival. In multivariate analysis, immunostaining of cathepsin D and EGFR obtained independent prognostic significance, and considered together (both negative, one positive, or both positive) was the strongest prognostic factor after tumor size.
Immunohistochemical staining for cathepsin D and EGFR is useful as a tool for evaluation of tumor aggressiveness in patients with early cervical squamous cell carcinoma.
本研究评估了组织蛋白酶D、表皮生长因子受体（EGFR）和c-erbB-2免疫组化染色在早期宫颈鳞状细胞癌患者中的预后意义。
这项回顾性分析纳入了132例患者，所有患者均因国际妇产科联盟（FIGO）I B期宫颈鳞状细胞癌接受了根治性子宫切除术及双侧盆腔淋巴结清扫术。免疫组化染色与各种组织病理学和形态学特征（即肿瘤大小、分化程度、血管浸润、宫旁组织浸润和淋巴结转移）以及无复发生存率相关。
47%的肿瘤组织蛋白酶D染色呈阳性，在发生淋巴结转移的肿瘤中更常见。组织蛋白酶D阳性肿瘤患者的无复发生存率较低。25.8%的肿瘤观察到EGFR过表达。与所研究的任何组织病理学变量均无相关性。EGFR过表达肿瘤患者的无复发生存率较低。12.1%的肿瘤观察到c-erbB-2免疫组化染色，与无复发生存率无关。在多变量分析中，组织蛋白酶D和EGFR的免疫染色具有独立的预后意义，综合考虑（两者均为阴性、一者为阳性或两者均为阳性）是仅次于肿瘤大小的最强预后因素。
组织蛋白酶D和EGFR免疫组化染色可作为评估早期宫颈鳞状细胞癌患者肿瘤侵袭性的工具。
