血管通透性因子（血管内皮生长因子）及其受体在子宫内膜癌中的表达

Expression of vascular permeability factor (vascular endothelial growth factor) and its receptors in endometrial carcinoma.

作者信息

Guidi A J, Abu-Jawdeh G, Tognazzi K, Dvorak H F, Brown L F

机构信息

Department of Pathology, Beth Israel Hospital and Harvard Medical School, Boston, Massachusetts, USA.

出版信息

Cancer. 1996 Aug 1;78(3):454-60. doi: 10.1002/(SICI)1097-0142(19960801)78:3<454::AID-CNCR12>3.0.CO;2-Y.

DOI:10.1002/(SICI)1097-0142(19960801)78:3<454::AID-CNCR12>3.0.CO;2-Y

PMID:8697391

Abstract

BACKGROUND

Solid tumors, including endometrial carcinomas, must induce a vascular stroma to grow beyond a minimal size. The mechanisms responsible for angiogenesis in endometrial carcinoma, however, are not well defined. Vascular permeability factor (VPF), also known as vascular endothelial growth factor (VEGF), is a multifunctional cytokine that is an important regulator of tumor angiogenesis. We evaluated VPF/VEGF mRNA and protein expression, as well as VPF/VEGF receptor mRNA expression, in endometrial carcinoma.

METHODS

Fourteen examples of endometrial carcinoma were evaluated by in situ hybridization; in 7 cases, benign atrophic endometrium from the same patient was also examined. Histologic sections were subjected to in situ hybridization using 35S-labeled riboprobes specific for VPF/VEGF and, in a subset of cases, riboprobes specific for the VPF/VEGF receptors flt-1 and KDR. In addition, ten examples of endometrial carcinoma were evaluated for VPE/VEGF protein expression by immunohistochemistry.

RESULTS

All 14 examples of endometrial carcinoma studied by in situ hybridization exhibited focal strong VPF/VEGF mRNA expression by tumor cells. In addition, the endothelial cells of surrounding microvessels strongly expressed flt-1 and KDR mRNAs in all ten cases examined. In contrast, no strong expression of VPF/VEGF, flt-1, or KDR mRNA was observed in the seven examples of benign atrophic endometrium studied. All ten cases of endometrial carcinoma studied by immunohistochemistry exhibited strong VPF/VEGF protein expression by tumor cells.

CONCLUSIONS

These observations suggest that VPF/VEGF is an important angiogenic factor in endometrial carcinoma.

摘要

背景

实体瘤，包括子宫内膜癌，必须诱导血管基质生长以超过最小尺寸。然而，子宫内膜癌中负责血管生成的机制尚未明确界定。血管通透性因子（VPF），也称为血管内皮生长因子（VEGF），是一种多功能细胞因子，是肿瘤血管生成的重要调节因子。我们评估了子宫内膜癌中VPF/VEGF mRNA和蛋白表达，以及VPF/VEGF受体mRNA表达。

方法

通过原位杂交评估了14例子宫内膜癌；在7例中，还检查了同一患者的良性萎缩性子宫内膜。组织切片使用针对VPF/VEGF的35S标记核糖探针进行原位杂交，在部分病例中，使用针对VPF/VEGF受体flt-1和KDR的核糖探针。此外，通过免疫组织化学评估了10例子宫内膜癌的VPE/VEGF蛋白表达。

结果

通过原位杂交研究的所有14例子宫内膜癌均显示肿瘤细胞有局灶性强VPF/VEGF mRNA表达。此外，在所检查的所有10例病例中，周围微血管的内皮细胞强烈表达flt-1和KDR mRNA。相比之下，在所研究的7例良性萎缩性子宫内膜中未观察到VPF/VEGF、flt-1或KDR mRNA的强表达。通过免疫组织化学研究的所有10例子宫内膜癌均显示肿瘤细胞有强VPF/VEGF蛋白表达。