Wilgenbus K K, Coy J F, Mincheva A, Nicolai H, Solomon E, Lichter P, Poustka A
Department of Molecular Analysis of the Genome, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
Cytogenet Cell Genet. 1996;73(3):240-3. doi: 10.1159/000134347.
Sixty-four PCR-markers previously assigned to the short arm of chromosome 17 and two newly established STSs were localized on a hybrid cell-YAC clone panel. The 66 STSs fell into 23 unique retention patterns, providing a map converting the entire short arm of human chromosome 17 with an average resolution of approximately 1.2 Mb. The combination of radiation-reduced hybrids, somatic cell hybrids and selected YAC clones enabled the precise localization of break-points in two cell hybrids. Since polymorphic STSs from the CEPH as well as the UTAH genetic map were used in this study, a physical link has been generated between these two high resolution genetic maps. FMR1L2, a second FMR1 autosomal homologue has been identified and assigned to a genomic interval between D17S796 and D17S799.
64个先前定位到17号染色体短臂上的PCR标记以及两个新建立的STS，被定位在一个杂种细胞 - YAC克隆板上。这66个STS可分为23种独特的保留模式，构建了一张平均分辨率约为1.2 Mb的人类17号染色体短臂图谱。辐射减少杂种、体细胞杂种和选定的YAC克隆相结合，实现了两个细胞杂种中断点的精确定位。由于本研究中使用了来自CEPH以及犹他州遗传图谱的多态性STS，因此在这两个高分辨率遗传图谱之间建立了物理联系。FMR1L2，即FMR1的第二个常染色体同源物，已被鉴定并定位到D17S796和D17S799之间的基因组区间。
