Zhao A, Li J, Lu Y
Department of Pathology, Beijing Institute for Cancer Research.
Zhonghua Zhong Liu Za Zhi. 1995 Sep;17(5):336-9.
The expression of P185erbB2 and P21ras was examined immunohistochemically in normal mucosa, intestinal metaplasia, dysplasia, intestinal type and diffuse type carcinoma of the stomach. The positive rates of P185 and P21 in the intestinal type carcinoma were significantly higher than those of the diffuse type. The results suggest that the two types of gastric carcinoma differ not only in histo-morphology but also in molecular mechanism. P21 immunopositivity is unlikely a specific marker of malignancy in gastric mucosa because of no significant difference of immunoreactivity among intestinal metaplasia, dysplasia, and carcinoma of the stomach. The positive staining of cell membrane for P185 was observed specifically in intestinal type carcinoma, but was absent in all other lesions. So it appears to be a useful marker of malignant change in the gastric mucosa. Overexpression of erbB2 gene in gastric carcinoma was demonstrated both at the mRNA level by in situ hybridization and at the protein level by immunohistochemical staining.
采用免疫组织化学方法检测了P185erbB2和P21ras在胃正常黏膜、肠化生、发育异常、肠型癌和弥漫型癌中的表达。肠型癌中P185和P21的阳性率显著高于弥漫型癌。结果表明，这两种类型的胃癌不仅在组织形态学上不同，而且在分子机制上也不同。P21免疫阳性不太可能是胃黏膜恶性肿瘤的特异性标志物，因为在肠化生、发育异常和胃癌之间免疫反应性没有显著差异。P185细胞膜阳性染色仅在肠型癌中特异性观察到，而在所有其他病变中均未出现。因此，它似乎是胃黏膜恶性变化的一个有用标志物。原位杂交在mRNA水平以及免疫组织化学染色在蛋白质水平均证实了erbB2基因在胃癌中的过表达。
