Ge L, Cao X, Ma D
Department of Immunology, Second Military Medical University, Shanghai.
Zhonghua Zhong Liu Za Zhi. 1995 Nov;17(6):409-11.
FBL-3 Leukemia cells transfected with IL-6 gene were expanded in vitro and inoculated into C57BL/6 mice subcutaneously. Tumor growth was observed and histologic analyses of the tumors in situ and the liver, spleen and bone marrow were performed at 14, 21, 28 and 35 days after inoculation. The mice inoculated with wild-type FBL-3 leukemia cells were used as the control. We found that the tumor invasiveness in the mice inoculated with FBL-3-IL-6+ occurred later than in the control group. The survival time of experimental mice was longer than in the control mice. The results demonstrated that inoculation of IL-6 high-secreting FBL-3 inhibited invasiveness of the leukemia cells, suggesting that the IL-6 gene transfected FBL-3 cells can be used as a vaccine to treat leukemia. The mechanism of the anti-invasiveness of IL-6 gene transfected leukemia cells needs further study.
将携带白细胞介素-6（IL-6）基因的FBL-3白血病细胞在体外扩增，然后皮下接种到C57BL/6小鼠体内。接种后14、21、28和35天观察肿瘤生长情况，并对原位肿瘤以及肝脏、脾脏和骨髓进行组织学分析。接种野生型FBL-3白血病细胞的小鼠作为对照。我们发现，接种FBL-3-IL-6+的小鼠肿瘤侵袭性比对照组出现得晚。实验小鼠的存活时间比对照小鼠长。结果表明，接种高分泌IL-6的FBL-3可抑制白血病细胞的侵袭性，提示转染IL-6基因的FBL-3细胞可作为治疗白血病的疫苗。转染IL-6基因的白血病细胞抗侵袭的机制需要进一步研究。
