Liang Y, Lu S, Guo Y
Cancer Institute, Chinese Academy of Medical Sciences, Beijing.
Zhonghua Zhong Liu Za Zhi. 1995 Nov;17(6):412-4.
Thirty-three esophageal tumors were analyzed by immunohistochemistry for P53 nuclear protein accumulation, and the results were compared to p53 gene mutations by PCR-direct sequence analysis. A highly significant correlation between the presence of p53 mutations and p53 nuclear protein accumulation was found. Of 33 tumors, 23 (69.7%) that demonstrated P53 protein expression 12(36%) had p53 mutations. Of 12 tumors with p53 mutations, 9 tumors showed P53 intensive nuclear reactivity. The results reported here are consistent with the idea that p53 mutations may be an important biological event in esophageal cancer progression and P53 expression was correlated with p53 gene mutation.
采用免疫组织化学方法分析了33例食管肿瘤中P53核蛋白的积聚情况，并通过PCR直接测序分析将结果与p53基因突变情况进行比较。发现p53基因突变与p53核蛋白积聚之间存在高度显著的相关性。在33例肿瘤中，有23例（69.7%）表现出P53蛋白表达，其中12例（36%）存在p53基因突变。在12例有p53基因突变的肿瘤中，9例显示出P53强核反应性。此处报道的结果与以下观点一致，即p53基因突变可能是食管癌进展中的一个重要生物学事件，且P53表达与p53基因突变相关。
