Manabe N, Imai Y, Ohno H, Takahagi Y, Sugimoto M, Miyamoto H
Department of Animal Science, Kyoto University, Japan.
Experientia. 1996 Jul 15;52(7):647-51. doi: 10.1007/BF01925566.
The porcine antral follicles, 3-6 mm in diameter, were dissected from the ovaries of mature pigs, and then granulosa and cumulus cells were isolated from each follicle. In atretic follicles, high activity of neutral Ca2+/Mg2+-dependent endonuclease and DNA ladder formation, estimated by electrophoresis, were noted in granulosa cells but not in cumulus cells. Extremely low activity of the endonuclease and no DNA ladder formation were observed in both types of cells obtained from healthy follicles. Moreover, apoptotic cells were observed histochemically among granulosa cells only. A good correlation (r = 0.987) between the endonuclease activity of granulosa cells and the progesterone/estradiol ratio of follicular fluid in each follicle was found. These results suggest that apoptosis occurs in granulosa cells but not cumulus cells in the atretic antral follicles in pigs.
从成年猪的卵巢中解剖出直径为3 - 6毫米的猪窦状卵泡，然后从每个卵泡中分离出颗粒细胞和卵丘细胞。在闭锁卵泡中，颗粒细胞中观察到中性Ca2+/Mg2+依赖性核酸内切酶的高活性以及通过电泳估计的DNA梯带形成，而卵丘细胞中未观察到。从健康卵泡获得的两种细胞中均观察到核酸内切酶的极低活性且无DNA梯带形成。此外，仅在颗粒细胞中通过组织化学观察到凋亡细胞。发现每个卵泡中颗粒细胞的核酸内切酶活性与卵泡液中孕酮/雌二醇比值之间存在良好的相关性（r = 0.987）。这些结果表明，猪闭锁窦状卵泡中的颗粒细胞发生凋亡，而卵丘细胞未发生凋亡。
