König J J, Teubel W, Romijn J C, Schröder F H, Hagemeijer A
Department of Urology, Erasmus University, Rotterdam, The Netherlands.
Hum Pathol. 1996 Jul;27(7):720-7. doi: 10.1016/s0046-8177(96)90404-9.
Fluorescence in situ hybridization (FISH) with centromere probes was used to investigate numerical aberrations of chromosomes 1, 7, 8, 10, 18, and Y in 46 prostate carcinoma (PC) and 11 benign prostatic hyperplasia (BPH) samples. None of the benign specimens showed any chromosomal aberration. Forty-one of 46 PC specimens showed numerical aberrations of one or more chromosomes. All investigated chromosomes showed numerical aberrations in at least 30% of the specimens, gain being more frequent than loss. Comparison of DNA flow cytometry (FCM) and FISH results showed that not only aneuploid tumors but also most diploid tumors harbored numerical chromosome aberrations. Chromosome 10 was the most frequently gained (65%), and Y the most frequently lost chromosome (14%). Nonmetastatic and metastatic tumors differed significantly (P < .05) in the number of copies for chromosomes 7, 8, and 10, but not for 1, 18, and Y. These results suggest strongly that gains of chromosomes 7, 8, and 10 are involved in PC progression.
采用着丝粒探针进行荧光原位杂交（FISH），以研究46例前列腺癌（PC）和11例良性前列腺增生（BPH）样本中1、7、8、10、18号染色体及Y染色体的数目畸变情况。所有良性样本均未显示任何染色体畸变。46例PC样本中有41例显示一条或多条染色体的数目畸变。所有研究的染色体在至少30%的样本中显示数目畸变，增加比丢失更常见。DNA流式细胞术（FCM）和FISH结果比较显示，不仅非整倍体肿瘤，而且大多数二倍体肿瘤也存在染色体数目畸变。10号染色体是最常增加的（65%），Y染色体是最常丢失的（14%）。非转移性和转移性肿瘤在7、8和10号染色体的拷贝数上有显著差异（P < 0.05），但在1、18号染色体及Y染色体上无差异。这些结果强烈提示，7、8和10号染色体的增加与PC进展有关。
