将野油菜黄单胞菌芝麻致病变种的胞外脂肪酶基因克隆至大肠杆菌。
Cloning of extracellular lipase gene from Xanthomonas campestris pathovar sesami on to Escherichia coli.
作者信息
Sheela P, Amuthan G, Mahadevan A
机构信息
Centre for Advanced Study in Botany, University of Madras.
出版信息
Indian J Exp Biol. 1996 Jan;34(1):27-31.
PMID:8698403
Abstract
A lipase gene from X. campestris pv. sesami (strain XcS 1) causal agent of leaf spot disease of Sesamum indicum, was cloned onto E. colt. XcS showed the presence of lip+ transformants on Dye's medium with 1% glycerol as sole carbon source. The recombinant plasmids were isolated and when digested with Eco R1, yielded 2 fragments with molecular weights 4.0 and 4.8 kb. Thus a 8.8 kb insert DNA fragment was obtained which showed lipase activity.
摘要
来自引起芝麻叶斑病的野油菜黄单胞菌芝麻致病变种（菌株XcS 1）的一个脂肪酶基因被克隆到大肠杆菌中。XcS在以1%甘油作为唯一碳源的戴氏培养基上显示出lip+转化体的存在。分离出重组质粒，用Eco R1消化时，产生了分子量分别为4.0和4.8 kb的两个片段。由此获得了一个显示脂肪酶活性的8.8 kb插入DNA片段。
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