Johnson C D, Reinitz C A, Sithigorngul P, Stretton A O
Department of Zoology, University of Wisconsin-Madison 53706, USA.
J Comp Neurol. 1996 Apr 8;367(3):352-60. doi: 10.1002/(SICI)1096-9861(19960408)367:3<352::AID-CNE3>3.0.CO;2-4.
We have used immunocytochemical techniques to investigate the distribution of serotonin-like immunoreactivity in the nematode Ascaris suum. Antisera raised against serotonin (5-hydroxytryptamine, 5-HT) conjugated to bovine serum albumin (BSA) labelled a pair of neurons in the pharynx of both sexes and five cells in the ventral cord of the male tail. The labelling was blocked by 5-HT or by 5-HT conjugated to BSA. The 5-HT-immunoreactive cells in the pharynx resemble neurosecretory cells and are probably homologous to the neurosecretory motor neurons (NSM) in Caenorhabditis elegans; the cells in the male tail appear to be motor neurons that are homologous to CP neurons in C. elegans. Other cells that stain with 5-HT antisera have been observed in C. elegans but are not seen in Ascaris.
我们运用免疫细胞化学技术研究了猪蛔虫体内类血清素免疫反应性的分布情况。针对与牛血清白蛋白（BSA）偶联的血清素（5-羟色胺，5-HT）制备的抗血清，标记了两性咽部的一对神经元以及雄性尾部腹索中的五个细胞。这种标记可被5-HT或与BSA偶联的5-HT阻断。咽部的5-HT免疫反应性细胞类似于神经分泌细胞，可能与秀丽隐杆线虫中的神经分泌运动神经元（NSM）同源；雄性尾部的细胞似乎是运动神经元，与秀丽隐杆线虫中的CP神经元同源。在秀丽隐杆线虫中观察到了其他被5-HT抗血清染色的细胞，但在猪蛔虫中未见到。
