Wang J B, Adler S P, Hempfling S, Burke R L, Duliège A M, Starr S E, Plotkin S A
Department of Pediatrics, Medical College of Virginia, Richmond 23298-0163, USA.
J Infect Dis. 1996 Aug;174(2):387-92. doi: 10.1093/infdis/174.2.387.
Because antibodies against human cytomegalovirus (HCMV) glycoprotein B (gB) neutralize, levels of IgG, secretory IgA (sIgA), and mucosal IgA1 antibodies to HCMV were measured in saliva and nasal washes. Ten seronegative adults lacked these antibodies, but of 10 naturally seropositive adults, 10 had IgG to gB, 5 had sIgA, and 0 had mucosal IgA. Among 12 recipients of a live HCMV vaccine, 8 had IgG to gB, 4 had sIgA, and 2 had mucosal IgA in samples collected 10-20 months after immunization; of 10 recipients of a gB vaccine, 8 had IgG to gB, 7 had sIgA, and 7 had mucosal IgA in samples collected just before or 1 month after a booster. IgG to gB and neutralizing titers in serum correlated with IgG to gB in mucosal samples. IgG to gB was in the saliva of 25 of 26 subjects with serum neutralizing titers > 1:64. Serum neutralizing titers > 1:64, whether induced by vaccine or wild type virus, are associated with mucosal IgG to HCMV.
由于抗人巨细胞病毒（HCMV）糖蛋白B（gB）的抗体具有中和作用，因此对唾液和鼻腔灌洗液中针对HCMV的IgG、分泌型IgA（sIgA）和黏膜IgA1抗体水平进行了检测。10名血清阴性的成年人缺乏这些抗体，但在10名自然血清阳性的成年人中，10人有针对gB的IgG，5人有sIgA，0人有黏膜IgA。在12名接种活HCMV疫苗的受种者中，免疫后10 - 20个月采集的样本中，8人有针对gB的IgG，4人有sIgA，2人有黏膜IgA；在10名接种gB疫苗的受种者中，加强免疫前或加强免疫后1个月采集的样本中，8人有针对gB的IgG，7人有sIgA，7人有黏膜IgA。血清中针对gB的IgG和中和滴度与黏膜样本中针对gB的IgG相关。26名血清中和滴度>1:64的受试者中，25人的唾液中有针对gB的IgG。血清中和滴度>1:64，无论由疫苗还是野生型病毒诱导，均与针对HCMV的黏膜IgG相关。
