Mehra V, Gong J H, Iyer D, Lin Y, Boylen C T, Bloom B R, Barnes P F
Department of Microbiology and Immunology, Albert Einstein College of Medicine, New York, New York, USA.
J Infect Dis. 1996 Aug;174(2):431-4. doi: 10.1093/infdis/174.2.431.
The capacity of four Mycobacterium tuberculosis recombinant antigens to elicit proliferation and cytokine production by human T cells was evaluated. Proliferative responses of peripheral blood mononuclear cells (PBMC) to all antigens were greater in healthy tuberculin reactors than in pulmonary tuberculosis patients, and proliferative responses of pleural fluid cells were greater than those of PBMC from patients with tuberculous pleuritis. The proliferative responses to the four recombinant antigens were similar in all patient groups, and there was no selective unresponsiveness to any antigen in pulmonary tuberculosis patients. The 38-kDa antigen induced less interferon-gamma than did the 10-, 30-, and 65-kDa antigens, and all four antigens induced similar amounts of interleukin-10. These results suggest that none of the four recombinant antigens are immunodominant, and that the 10-, 30-, and 65-kDa antigens are similar in their capacity to induce a potentially protective Th1-like response.
评估了四种结核分枝杆菌重组抗原激发人T细胞增殖和细胞因子产生的能力。健康结核菌素反应者外周血单个核细胞（PBMC）对所有抗原的增殖反应大于肺结核患者，胸腔积液细胞的增殖反应大于结核性胸膜炎患者的PBMC。所有患者组对四种重组抗原的增殖反应相似，肺结核患者对任何抗原均无选择性无反应性。38 kDa抗原诱导的干扰素-γ少于10 kDa、30 kDa和65 kDa抗原，且所有四种抗原诱导的白细胞介素-10量相似。这些结果表明，四种重组抗原均无免疫优势，且10 kDa、30 kDa和65 kDa抗原在诱导潜在保护性Th1样反应的能力方面相似。
