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脱氧血红蛋白和配体结合血红蛋白中游离氨基和结合氨基的不同反应活性。

Different reactivities of free and bound amino groups in deoxy-and liganded haemoglobin.

作者信息

Bresciani D

出版信息

Biochem J. 1977 May 1;163(2):393-5. doi: 10.1042/bj1630393.

DOI:10.1042/bj1630393
PMID:869933
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1164710/
Abstract

The technique of competitive double-labelling [H. Kaplan, K.J. Stevenson & B.S. Hartley, (1971) Biochem. J. 124, 289-299; L.P. Visentin & H. Kaplan (1975) Biochemistry 14, 463-468] was used to determine the reactivity of some amino groups towards acetic anhydride in deoxy-and liganded haemoglobin. Only those amino groups known to form salt bridges in deoxy-but not in liganded haemoglobin (i.e. the alpha-amino group of valine-1 alpha and the xi-amino group of lysine-40 alpha and lysine-127 alpha [M. F. Perutz (1970) Nature (London) 228, 726-739]) and different reactivities in the two structures.

摘要

采用竞争性双标记技术[H. 卡普兰、K.J. 史蒂文森和B.S. 哈特利，(1971年)《生物化学杂志》124卷，289 - 299页；L.P. 维森廷和H. 卡普兰(1975年)《生物化学》14卷，463 - 468页]来测定脱氧血红蛋白和配体血红蛋白中某些氨基与乙酸酐的反应活性。只有那些已知在脱氧血红蛋白中形成盐桥而在配体血红蛋白中不形成盐桥的氨基(即缬氨酸-1α的α-氨基以及赖氨酸-40α和赖氨酸-127α的ξ-氨基[M.F. 佩鲁茨(1970年)《自然》(伦敦)228卷，726 - 739页])，并且在两种结构中具有不同的反应活性。

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Acetylation of amino and tyrosine hydroxyl groups. Preparation of inhibitors of oxytocin with no intrinsic activity on the isolated uterus.氨基和酪氨酸羟基的乙酰化。制备对离体子宫无内在活性的催产素抑制剂。
J Biol Chem. 1967 Apr 10;242(7):1592-8.
2
Stereochemistry of cooperative effects in haemoglobin.血红蛋白协同效应的立体化学
Nature. 1970 Nov 21;228(5273):726-39. doi: 10.1038/228726a0.
3
The binding of carbon dioxide by horse haemoglobin.马血红蛋白对二氧化碳的结合作用。
Biochem J. 1971 Aug;124(1):31-45. doi: 10.1042/bj1240031.
4
Competitive labelling, a method for determining the reactivity of individual groups in proteins. The amino groups of porcine elastase.竞争性标记，一种确定蛋白质中各个基团反应性的方法。猪弹性蛋白酶的氨基。
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5
Three-dimensional fourier synthesis of human deoxyhaemoglobin at 2-5 A resolution: refinement of the atomic model.2-5埃分辨率下人类脱氧血红蛋白的三维傅里叶合成：原子模型的精修
J Mol Biol. 1975 Sep 15;97(2):237-56. doi: 10.1016/s0022-2836(75)80037-4.
6
A chemical approach to the fine structure of biomolecular complexes: the amino terminal region of the 50S ribosomal "A" protein from Bacillus stearothermophilus.一种研究生物分子复合物精细结构的化学方法：嗜热脂肪芽孢杆菌50S核糖体“A”蛋白的氨基末端区域
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