基于具有疏水基团和离子基团吸附剂的蛋白质色谱法。人红细胞血型糖蛋白的纯化。
Protein chromatography on adsorbents with hydrophobic and ionic groups. Purification of human erythrocyte glycophorin.
作者信息
Simmonds R J, Yon R J
出版信息
Biochem J. 1977 May 1;163(2):397-400. doi: 10.1042/bj1630397.
Abstract
Human erythrocyte glycophorin was purified rapidly by (a) chromatography of a Triton X-100 extract of erythrocyte 'ghosts' on N-(3-carboxypropionyl)aminodecyl-Sepharose in buffers containing Triton X-100 or sodium dodecyl sulphate, or (b) chromatography of whole 'ghosts', solubilized in sodium dodecyl sulphate, on dodecyl-Sepharose, in buffers containing sodium dodecyl sulphate. The products contained 85-95% glycophorin (electrophoretic band PAS-1) and the major contaminants were glycoproteins PAS-2 (possibly a subunit of glycophorin) and PAS-3.
摘要
人红细胞血型糖蛋白可通过以下方法快速纯化
(a) 在含有 Triton X-100 或十二烷基硫酸钠的缓冲液中，将红细胞“血影”的 Triton X-100 提取物在 N-(3-羧基丙酰基)氨基十二烷基-琼脂糖上进行层析；或(b) 将溶解在十二烷基硫酸钠中的完整“血影”在含有十二烷基硫酸钠的缓冲液中的十二烷基-琼脂糖上进行层析。产物中含有 85 - 95%的血型糖蛋白（电泳带 PAS-1），主要污染物是糖蛋白 PAS-2（可能是血型糖蛋白的一个亚基）和 PAS-3。
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2
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J Biol Chem. 1969 Aug 25;244(16):4406-12.
3
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4
Selective solubilization of proteins and phospholipids from red blood cell membranes by nonionic detergents.非离子去污剂对红细胞膜中蛋白质和磷脂的选择性增溶作用。
J Supramol Struct. 1973;1(3):233-48. doi: 10.1002/jss.400010308.
5
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6
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