Roby K F, Laham N, Hunt J S
Department of Anatomy, University of Kansas Medical Center, Kansas City 66160-7400, USA.
J Reprod Fertil. 1996 Mar;106(2):285-90. doi: 10.1530/jrf.0.1060285.
Signals transduced by binding of tumour necrosis factor alpha (TNF) and lymphotoxin alpha (LT-alpha) trimers to high-affinity cell membrane receptors, TNF-RI (p55/p60) and TNF-RII (p75/p80), affect many cell functions. In this study, expression of the gene encoding TNF-RI in uteri of cyclic mice was mapped using in situ hybridization. TNF-RI hybridization signals fluctuated during the cycle. Signal intensity was highest during dioestrus-II, when mRNA encoding TNF-RI was present in endometrial epithelial and stroma cells, as well as in myometrial smooth muscle and connective tissue cells. The ability of oestradiol and progesterone to modulate steady state concentrations of mRNA encoding TNF-RI in uterine cells was assessed by using in situ and northern blot hybridization procedures. Seven days after ovariectomy, low concentrations of mRNA encoding TNF-RI were detected by northern analysis and weak in situ hybridization signals were identified in epithelia and some myometrial connective tissue cells. Administration of oestradiol, progesterone or oestradiol plus progesterone to ovariectomized animals stimulated temporal and cell type-specific changes in steady state concentrations of mRNA encoding TNF-RI that were unique to each hormonal regimen. Maximal induction of mRNA encoding TNF-RI required 24 h of oestradiol stimulation and 72 h of progesterone stimulation. In uteri treated with oestradiol plus progesterone, the oestradiol pattern predominated over the progesterone pattern. Thus, multiple cell types in cyclic mouse uteri express the gene encoding TNF-RI, and expression in specific cells is controlled by female steroid hormones.
肿瘤坏死因子α（TNF）和淋巴毒素α（LT-α）三聚体与高亲和力细胞膜受体TNF-RI（p55/p60）和TNF-RII（p75/p80）结合所转导的信号，影响许多细胞功能。在本研究中，使用原位杂交技术对周期性小鼠子宫中编码TNF-RI的基因表达进行了定位。TNF-RI杂交信号在周期中波动。信号强度在动情后期-II最高，此时编码TNF-RI的mRNA存在于子宫内膜上皮细胞、基质细胞、子宫肌层平滑肌细胞和结缔组织细胞中。通过原位杂交和Northern印迹杂交方法评估雌二醇和孕酮调节子宫细胞中编码TNF-RI的mRNA稳态浓度的能力。卵巢切除术后7天，Northern分析检测到编码TNF-RI的mRNA浓度较低，原位杂交信号较弱，仅在上皮细胞和一些子宫肌层结缔组织细胞中检测到。对去卵巢动物给予雌二醇、孕酮或雌二醇加孕酮，可刺激编码TNF-RI的mRNA稳态浓度发生时间和细胞类型特异性变化，每种激素方案的变化都是独特的。编码TNF-RI的mRNA的最大诱导需要24小时的雌二醇刺激和72小时的孕酮刺激。在用雌二醇加孕酮处理的子宫中，雌二醇模式占主导地位。因此，周期性小鼠子宫中的多种细胞类型表达编码TNF-RI的基因，并且特定细胞中的表达受雌性甾体激素控制。
