Andersen U O, Bøg-Hansen T C, Kirkeby S
Faculty of Health Sciences, University of Copenhagen, Denmark.
J Reprod Fertil. 1996 May;107(1):11-6. doi: 10.1530/jrf.0.1070011.
A histochemical avidin-biotin technique with three different alpha 1-acid glycoprotein glycoforms showed pronounced alterations in the cellular localization of two alpha 1-acid glycoprotein lectin-like receptors during cell differentiation in the developing rat testis. The binding of alpha 1-acid glycoprotein glycoforms to their receptors is inhibited by steroids. Testosterone, oestradiol and progesterone inhibited the binding of alpha 1-acid glycoprotein glycoform A to its receptor. Cortisone, aldosterone, oestradiol and progesterone inhibited the binding of alpha 1-acid glycoprotein glycoforms B and C to their receptor. A difference in the cellular content of alpha 1-acid glycoprotein glycoforms and alpha 1-acid glycoprotein receptors separates the spermatocytes and the early spermatids from the late spermatids. The difference in receptor composition implies a difference in the effect of different steroid hormones. The Leydig cells contained alpha 1-acid glycoprotein and lectin-like receptors for one of the glycoforms of alpha 1-acid glycoprotein from birth. The interaction between alpha 1-acid glycoprotein glycoforms and their receptors may modulate the actions of testosterone and other steroids in the testis.
一种采用三种不同α1-酸性糖蛋白糖型的组织化学抗生物素蛋白-生物素技术显示，在发育中的大鼠睾丸细胞分化过程中，两种α1-酸性糖蛋白凝集素样受体的细胞定位发生了显著变化。α1-酸性糖蛋白糖型与其受体的结合受到类固醇的抑制。睾酮、雌二醇和孕酮抑制α1-酸性糖蛋白糖型A与其受体的结合。可的松、醛固酮、雌二醇和孕酮抑制α1-酸性糖蛋白糖型B和C与其受体的结合。α1-酸性糖蛋白糖型和α1-酸性糖蛋白受体的细胞含量差异将精母细胞和早期精子细胞与晚期精子细胞区分开来。受体组成的差异意味着不同类固醇激素的作用存在差异。从出生起，睾丸间质细胞就含有α1-酸性糖蛋白和α1-酸性糖蛋白一种糖型的凝集素样受体。α1-酸性糖蛋白糖型与其受体之间的相互作用可能会调节睾丸中睾酮和其他类固醇的作用。
