与雄性孤星蜱（蜱螨亚纲：硬蜱科）叮咬相关的唾液腺变化和宿主抗体反应。
Salivary gland changes and host antibody responses associated with feeding of male lone star ticks (Acari:Ixodidae).
作者信息
Sanders M L, Scott A L, Glass G E, Schwartz B S
机构信息
Johns Hopkins University, School of Hygiene and Public Health, Baltimore, MD 21205, USA.
出版信息
J Med Entomol. 1996 Jul;33(4):628-34. doi: 10.1093/jmedent/33.4.628.
The goal of this study was to demonstrate that male lone star ticks, Amblyomma americanum (L.), actively feed on rabbits during attachment and that the host is capable of mounting an immune response against male salivary gland proteins. During attachment, it was shown that male ticks salivary glands hypertrophy. An enzyme-linked immunosorbent assay was used to detect rabbit serum proteins in the midgut of previously attached male ticks. SDS-polyacrylamide gel electrophoresis showed that the proteins expressed in the male tick salivary gland changed during feeding, with several new proteins in the 15- to 50-kDa range synthesized during attachment. Rabbits mounted a detectable antibody response against male tick salivary gland proteins after 2 sequential feedings of male ticks. The antibodies were directed against a spectrum of male salivary gland proteins ranging from 18 to 160 kDa. Several of these proteins were not recognized by antibodies directed against female tick saliva proteins, and thus may be specific male salivary gland components. This evidence indicates that male A. americanum ticks actively feed during attachment, that their salivary gland proteins change during feeding, and that male salivary gland proteins are immunogenic.
本研究的目的是证明雄性美洲钝眼蜱（Amblyomma americanum (L.)）在附着过程中会主动吸食兔子血液，且宿主能够对雄性唾液腺蛋白产生免疫反应。在附着过程中，发现雄性蜱的唾液腺会肥大。采用酶联免疫吸附测定法检测先前附着的雄性蜱中肠内的兔血清蛋白。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示，雄性蜱唾液腺中表达的蛋白质在取食过程中发生了变化，在附着期间合成了几种15至50 kDa范围内的新蛋白质。在连续两次喂食雄性蜱后，兔子对雄性蜱唾液腺蛋白产生了可检测到的抗体反应。这些抗体针对一系列18至160 kDa的雄性唾液腺蛋白。其中几种蛋白质未被针对雌性蜱唾液蛋白的抗体识别，因此可能是雄性唾液腺的特异性成分。这一证据表明，雄性美洲钝眼蜱在附着过程中会主动取食，其唾液腺蛋白在取食过程中会发生变化，且雄性唾液腺蛋白具有免疫原性。
Zotero 插件