Giorgio A, De Falco M L
Quad Sclavo Diagn. 1978 Jun;14(2):284-8.
The haematological method (Giemsa, Pappenheim, Wright, ecc.) is renowed for karyological legible, while the cytologic method (Papanicolau, Shorr, haematoxylon-eosine, etc.) for its view of the whole. Antithesis between two methods was subject polemics and discussions after put in round table promoted by Acta cytological in 1958. The present work suggests for the attention of cytologists an attempt at technical reconciliation of two methods, it obtained with a new coloration method that uses like fixative a cadmiumalcoholacetic mixture, like nuclear colouring the basic fuchsine maturing in presence of formic aldehyde and acetic acid, like cytoplasmatic colouring a triacid orange-eosine-blue mixture of aniline.
血液学方法（吉姆萨、帕彭海姆、赖特等）以核型清晰可读而闻名，而细胞学方法（巴氏、肖尔、苏木精 - 伊红等）则以能观察整体而著称。1958年《细胞学报》组织的一次圆桌会议引发了两种方法之间的对立，引发了诸多争论和讨论。本研究向细胞学家们介绍一种尝试，即通过一种新的染色方法对两种方法进行技术上的调和。该方法使用镉 - 酒精 - 乙酸混合物作为固定剂，用在甲醛和乙酸存在下成熟的碱性品红作为核染色剂，用苯胺的三酸橙 - 伊红 - 蓝混合物作为细胞质染色剂。
