Swindle M M, Wiest D B, Smith A C, Garner S S, Case C C, Thompson R P, Fyfe D A, Gillette P C
Department of Comparative Medicine, Medical University of South Carolina, Charleston, SC 29425-2211, USA.
Lab Anim Sci. 1996 Feb;46(1):90-5.
Thirty-nine Yucatan miniature swine were used in three fetal surgical experimental protocols. They involved antiarrhythmic administration, pacemaker implantation, and in-utero diagnosis of ventricular septal defect by intraoperative echocardiography. Because of problems encountered with surgical protocols in the initial stages, modifications were made to prevent fetal hypothermia and intraoperative mortality. These modifications included environmental temperature support, staple surgical techniques to reduce operative time, and development of fetal catheters designed to facilitate cannulation of small vessels. Postoperative care protocols were intensive and included antibiotics, analgesics, and supportive care designed to reduce discomfort and prevent abortion and sepsis. Thirty-seven of 39 sows survived the surgical procedures; experiments were performed on 117 fetuses. Twenty-two fetuses died either intraoperatively or postoperatively because of complications related to the experimental protocols. Modification of surgical and postsurgical protocols for these projects demonstrates the feasibility of using miniature swine as a model for fetal surgery, when their use was appropriate for anatomic and physiologic reasons.
39只尤卡坦小型猪被用于三个胎儿外科实验方案。这些方案包括抗心律失常药物给药、起搏器植入以及通过术中超声心动图对室间隔缺损进行宫内诊断。由于在初始阶段手术方案中遇到问题，因此进行了修改以防止胎儿体温过低和术中死亡。这些修改包括环境温度支持、采用吻合器手术技术以减少手术时间，以及开发旨在便于对小血管进行插管的胎儿导管。术后护理方案十分严格，包括使用抗生素、镇痛药以及旨在减轻不适并防止流产和败血症的支持性护理。39只母猪中有37只在手术过程中存活下来；对117只胎儿进行了实验。22只胎儿因与实验方案相关的并发症在术中或术后死亡。对这些项目的手术和术后方案进行修改表明，当出于解剖学和生理学原因适合使用小型猪时，将其作为胎儿手术模型是可行的。
