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β-乳球蛋白与氯化胍和尿素等温相互作用的热力学

Thermodynamics of the isothermal interaction of beta-lactoglobulin with guanidinium chloride and urea.

作者信息

Lapanje S, Lunder M, Vlachy V, Skerjanc J

出版信息

Biochim Biophys Acta. 1977 Apr 25;491(2):482-90. doi: 10.1016/0005-2795(77)90291-4.

Abstract

A thermodynamic study of the isothermal interaction of beta-lactoglobulin with guanidinium chloride and urea has been performed. Enthalpies of interaction of the two denaturants have been obtained by calorimetric measurements, and the free energy of interaction calculated from previously determined preferential binding of denaturants. In separate dilatometric experiments the volume changes accompanying the interaction of guanidinium chloride with beta-lactoglobulin have also been determined.

摘要

已对β-乳球蛋白与氯化胍和尿素的等温相互作用进行了热力学研究。通过量热测量获得了两种变性剂的相互作用焓,并根据先前测定的变性剂优先结合情况计算了相互作用自由能。在单独的膨胀计实验中,还测定了氯化胍与β-乳球蛋白相互作用时伴随的体积变化。

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