Murray B, Shizgal P
Department of Psychology, Concordia University, Montréal, Qué., Canada.
Physiol Behav. 1996 Mar;59(3):427-37. doi: 10.1016/0031-9384(95)02077-2.
Extracellular recordings were obtained, in urethane-anesthetized rats, from 44 neurons in the rostral bed nuclei of the medial forebrain bundle (MFB). These cells were antidromically activated by stimulation of MFB sites that typically support self-stimulation. Recording sites included the magnocellular preoptic nucleus, substantia innominata, ventral pallidum, olfactory tubercle, and horizontal limb of the diagonal band. Refractory period estimates ranged from 0.35 to 1.20 ms (mean +/- SD = 0.72 +/- 0.30 ms, n = 15) for stimulation sites in the lateral hypothalamic and ventral tegmental areas when using currents of twice threshold and procedures designed to estimate excitability at or near the site of stimulation. Interelectrode conduction velocity estimates ranged from 1.48 to 20.0 m/s (mean +/- SD = 9.26 +/- 7.22 m/s, n = 11) and were obtained by dividing the interelectrode distance by the difference in the response latency from the two MFB stimulation sites. The refractory period and conduction velocity estimates for these neurons overlap the psychophysically derived estimates for MFB reward neurons. These data are consistent with the hypothesis that neurons arising in the rostral bed nucleus of the MFB compose at least part of the directly activated substrate for MFB self-stimulation.
在乌拉坦麻醉的大鼠中，从内侧前脑束（MFB）吻侧床核的44个神经元进行了细胞外记录。这些细胞通过刺激通常支持自我刺激的MFB部位而被逆向激活。记录部位包括大细胞视前核、无名质、腹侧苍白球、嗅结节和斜角带水平支。当使用两倍阈值电流和旨在估计刺激部位或其附近兴奋性的程序时，外侧下丘脑和腹侧被盖区刺激部位的不应期估计范围为0.35至1.20毫秒（平均值±标准差=0.72±0.30毫秒，n = 15）。电极间传导速度估计范围为1.48至20.0米/秒（平均值±标准差=9.26±7.22米/秒，n = 11），通过将电极间距离除以来自两个MFB刺激部位的反应潜伏期差异获得。这些神经元的不应期和传导速度估计值与MFB奖赏神经元的心理物理学推导估计值重叠。这些数据与以下假设一致，即起源于MFB吻侧床核的神经元至少构成了MFB自我刺激的直接激活底物的一部分。
