Houpt T A, Frankmann S P, Berlin R
E. W. Bourne Behavioral Research Laboratory, Department of Psychiatry, Cornell University Medical College, White Plains, NY 10605, USA.
Physiol Behav. 1996 Mar;59(3):495-8. doi: 10.1016/0031-9384(95)02090-x.
Electrophysiological and biochemical evidence suggests that cAMP mediates sweet taste transduction. Neural recordings from anesthetized rats and in vitro preparations demonstrate that membrane-per-meable cAMP analogues mimic the effects of sucrose and artificial sweetners. We presented solutions of sodium 8-(4-chlorophenylthio)-adenosine 3'-5'-cyclic monophosphate (8cpt-cAMP), a water-soluble, membrane-permeable cAMP analogue to freely behaving rats in short-term lickometer tests. Rats licked significantly less to 8cpt-cAMP than to sucrose or palatable saccharin solutions. Rats could taste 8cpt-cAMP solutions, however, because they licked less to 8cpt-cAMP in mixture with sucrose than to sucrose alone. Because 8cpt-cAMP decreased licking when mixed with sucrose, we conclude that the taste of 8cpt-cAMP is aversive, not sweet, to freely behaving rats.
电生理和生化证据表明，环磷酸腺苷（cAMP）介导甜味转导。对麻醉大鼠和体外制剂的神经记录表明，可透过细胞膜的cAMP类似物模拟了蔗糖和人工甜味剂的作用。在短期舔舐计测试中，我们将8-（4-氯苯硫基）-腺苷3'-5'-环一磷酸钠（8cpt-cAMP，一种水溶性、可透过细胞膜的cAMP类似物）溶液呈现给自由活动的大鼠。与蔗糖或可口的糖精溶液相比，大鼠对8cpt-cAMP的舔舐显著减少。然而，大鼠能够尝出8cpt-cAMP溶液的味道，因为与单独的蔗糖相比，它们对与蔗糖混合的8cpt-cAMP的舔舐更少。由于8cpt-cAMP与蔗糖混合时会减少舔舐，我们得出结论，对于自由活动的大鼠来说，8cpt-cAMP的味道是厌恶的，而不是甜的。
