Wikström L M, Meister B, Franck J, Fried G
Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden.
Regul Pept. 1996 Jan 16;61(1):37-44. doi: 10.1016/0167-0115(95)00134-4.
Enkephalin-like immunoreactivity (ENK-LI), neuropeptide Y (NPY)-LI and dopamine-beta-hydroxylase (DBH)-LI were found within the chromaffin cells of both the paraaortic body and the adrenal medulla of the newborn rabbit using immunohistochemistry. Cells positive to DBH-LI were abundant in both the paraaortic body and the adrenal medulla. ENK-LI positive cells were frequent in the paraaortic body, but more sparse in the adrenal medulla. A few cells staining for NPY-LI could be detected in both organs. Some nerve fibers within these organs also contained substance P-LI and calcitonin-gene related peptide-LI. The tissue contents of ENK-LI and NPY-LI, as measured by radioimmunoassay, increased after birth in the adrenal glands and were significantly higher than the fetal levels from 1 week of age. In the paraaortic body the lowest content of ENK-LI was found around birth, whereas the content of NPY-LI was highest at that time. With advancing postnatal age, the content of ENK-LI increased, whereas the content of NPY-LI decreased. At each age, there was a higher content of ENK-LI as compared to NPY-LI in both organs. This indicates that the synthesis of ENK-LI and NPY-LI in the paraaortic body is differently regulated during perinatal development.
采用免疫组织化学方法，在新生兔的主动脉旁体和肾上腺髓质的嗜铬细胞内发现了脑啡肽样免疫反应性物质（ENK-LI）、神经肽Y（NPY）-LI和多巴胺-β-羟化酶（DBH）-LI。DBH-LI阳性细胞在主动脉旁体和肾上腺髓质中均很丰富。ENK-LI阳性细胞在主动脉旁体中较为常见，但在肾上腺髓质中则较为稀疏。在这两个器官中均可检测到少数NPY-LI染色阳性的细胞。这些器官内的一些神经纤维也含有P物质-LI和降钙素基因相关肽-LI。通过放射免疫测定法测得，肾上腺中ENK-LI和NPY-LI的组织含量在出生后增加，且从1周龄起显著高于胎儿水平。在主动脉旁体中，ENK-LI的含量在出生前后最低，而NPY-LI的含量在此时最高。随着出生后年龄的增长，ENK-LI的含量增加，而NPY-LI的含量降低。在每个年龄段，两个器官中ENK-LI的含量均高于NPY-LI。这表明在围产期发育过程中，主动脉旁体中ENK-LI和NPY-LI的合成受到不同的调节。
