Pershina M Iu, Tolovskaia K R, Akatov A K, Khorobrykh V V, Pavlova I P, Skvortsov V T
Zh Mikrobiol Epidemiol Immunobiol. 1996 Mar-Apr(2):64-7.
Highly purified teichoic acids (TA) were isolated from Staphylococcus aureus and S. epidermidis and characterized. The preparations of TA were highly species-specific. For the first time monospecific sera to S. aureus and S. epidermidis were obtained. From these monospecific sera immunoglobulins were isolated and used for the preparation of reagents for the passive hemagglutination (PHA) test and the enzyme immunoassay (EIA). The sensitivity of the PHA test and EIA was 15 micrograms/ml when S. aureus and S. epidermidis were used and 10(6)-10(7) cells/ml when whole microbial cells were used. The diagnostic preparations proved to be highly specific and did not react with other preparations isolated from S. aureus and S. epidermidis, as well as from bacteria belonging to other taxa. Experiments on rabbits, carried out with the use of newly developed test systems, demonstrated that staphylococci could be detected in the blood as early as 10 minutes after the intravenous infection of the animals and until day 12.
从金黄色葡萄球菌和表皮葡萄球菌中分离并鉴定了高度纯化的磷壁酸（TA）。TA制剂具有高度的种特异性。首次获得了针对金黄色葡萄球菌和表皮葡萄球菌的单特异性血清。从这些单特异性血清中分离出免疫球蛋白，并用于制备被动血凝试验（PHA）和酶免疫测定（EIA）的试剂。当使用金黄色葡萄球菌和表皮葡萄球菌时，PHA试验和EIA的灵敏度为15微克/毫升，当使用全微生物细胞时，灵敏度为10⁶ - 10⁷个细胞/毫升。诊断制剂被证明具有高度特异性，并且不与从金黄色葡萄球菌和表皮葡萄球菌以及其他分类群的细菌中分离出的其他制剂发生反应。使用新开发的测试系统对兔子进行的实验表明，在动物静脉感染后10分钟即可在血液中检测到葡萄球菌，并且一直持续到第12天。
