免疫球蛋白G的分子结构改变增强了静脉注射免疫球蛋白的抗补体活性。

Molecular structure alteration of IgG increased anticomplementary activity of intravenous immunoglobulin.

作者信息

Zang X X, Sun Y, Shen F, Wang D, Zhou L, Wang J, Kung E

机构信息

Department of Quality Control, Shanghai RAAS Blood Products Co Ltd, China.

出版信息

Zhongguo Yao Li Xue Bao. 1995 Sep;16(5):415-9.

PMID:8701757

Abstract

AIM

To study the cause of anticomplementary activities (ACA) of intravenous immunoglobulins (IVIG).

METHODS

ACA were determined by two assays (limit test and 100CH50 test), distributions of IgG molecular size (polymer, dimer, monomer and fragment) by HPLC, IgG subclasses and IgA contents by radial immunodiffusion, prekallikrein activator (PKA) and kallikrein (KK) activities by chromogenic assay, potency of antibody against hepatitis B surface antigen (Anti-HBs) by enzyme-linked immunosorbent assay (ELISA).

RESULTS

The two ACA assays showed good correlation. However, ACA levels were not associated with distributions of IgG molecular size, IgG subclasses and IgA contents, PKA and KK activities. After heating incubation, ACA levels increased markedly and Anti-HBs decreased notably, distributions of IgG molecular size remained relatively constant.

CONCLUSION

Molecular structure alteration of IgG increased spontaneous complement activation of IVIG.

摘要

目的

研究静脉注射免疫球蛋白（IVIG）抗补体活性（ACA）的原因。

方法

采用两种检测方法（限度试验和100CH50试验）测定ACA，通过高效液相色谱法（HPLC）测定IgG分子大小（聚合物、二聚体、单体和片段）的分布，通过放射免疫扩散法测定IgG亚类和IgA含量，通过显色法测定前激肽释放酶激活剂（PKA）和激肽释放酶（KK）活性，通过酶联免疫吸附测定法（ELISA）测定抗乙型肝炎表面抗原抗体（抗-HBs）的效价。