[Microdialysis sampling and microbore liquid chromatography with electrochemical detection for determination of serotonin in rat brain striatum].

AIM

To develop a sensitive method for determination of serotonin in biological samples.

METHODS

A combination of microdialysis sampling and microbore liquid chromatography with electrochemical detection (LCEC) was established.

RESULTS

Changes of serotonin in fg or pg in microdialysates from brain striatum of the free moving rat were easily determined.

CONCLUSION

This developed method was useful for living animal research. Serotonin level in corpus striatum healthy rats was quite stable.