Cantin G, Delage R, Roy J, Marchand-Laroche D, Leblond P F
Centre d'hématologie et d'immunologie clinique, Hôpital du Saint-Sacrement, Quebec, Canada.
Bone Marrow Transplant. 1996 Mar;17(3):347-50.
Three patients received sequential transfusions of G-CSF-mobilized peripheral blood stem cells from their identical twin in an attempt to abrogate neutropenia. Blood stem cells were harvested by leukapheresis in the healthy donor twins following mobilization with rhG-CSF at 5 micrograms/kg/day subcutaneously for at least 5 days. An average of 2.2 x 10(7) CFU-GM (range: 1.4-3.3) were collected and transfused without further manipulation. One patient, transfused with a CFU-GM dose of 3 x 10(7) on day +6 after a syngeneic marrow transplant, experienced near-complete elimination of absolute neutropenia until spontaneous engraftment occurred on day +11. In the other two patients, we unexpectedly observed a transient granulopoietic inhibition, possibly related to the high T cell content of the blood stem cell transfusions.
三名患者接受了来自同卵双胞胎的经粒细胞集落刺激因子（G-CSF）动员的外周血干细胞的序贯输注，试图消除中性粒细胞减少症。在健康供体双胞胎中，皮下注射重组人粒细胞集落刺激因子（rhG-CSF），剂量为5微克/千克/天，持续至少5天，然后通过白细胞分离术采集血液干细胞。平均采集了2.2×10⁷集落形成单位-粒细胞巨噬细胞（CFU-GM）（范围：1.4 - 3.3），未经进一步处理就进行了输注。一名患者在同基因骨髓移植后第+6天接受了3×10⁷的CFU-GM剂量输注，在第+11天自发植入前，绝对中性粒细胞减少症几乎完全消除。在另外两名患者中，我们意外地观察到短暂的粒细胞生成抑制，这可能与血液干细胞输注中高T细胞含量有关。
