Ridley R K, Slonka G F, Leland S E
J Parasitol. 1977 Apr;63(2):348-56.
Cultures of Cooperia punctata, a nematode parasitic in cattle, were studied in Ae medium at a pH of 7.2 to 7.3 under air. In fourth and fifth stages of development, they absorbed or otherwise took into their free pool and tissues, carbon derived from 14C-labeled sodium propionate and converted, by pathways not elucidated, a portion of this carbon into protein and lipid fractions. Thirty minutes postincubation in balanced slat solution was adequate to reduce the amount of isotope in the nematode gut to a constant level. Activity from specifically-labeled 14C-propionate was recovered (as glucose pentaacetate) from worm glucose and from CO2 evolved from cultures consisting of L4 and adult stages. Use of propionate by these worms, for whatever metabolic purpose, would result in depriving the ruminant host of some of its necessary glucogenic precursors, and could account for a specific pathogenic mechanism attendant to heavy infections with this parasite.
对寄生于牛的点状古柏线虫（Cooperia punctata）在空气环境下、pH为7.2至7.3的Ae培养基中进行培养研究。在发育的第四和第五阶段，它们吸收或以其他方式将源自14C标记的丙酸钠的碳摄入其自由池和组织中，并通过未阐明的途径将一部分碳转化为蛋白质和脂质部分。在平衡盐溶液中孵育30分钟足以将线虫肠道中的同位素量降低至恒定水平。从蠕虫葡萄糖以及由L4期和成虫期组成的培养物中释放的二氧化碳中回收了来自特异性标记的14C-丙酸盐的活性（以葡萄糖五乙酸酯形式）。这些蠕虫使用丙酸盐，无论出于何种代谢目的，都会导致反刍动物宿主失去一些必需的生糖前体，并可能解释这种寄生虫重度感染时伴随的特定致病机制。
