Chen W Y, Lu C C
Department of Pediatrics, Kaohsiung Medical College, Taiwan, Republic of China.
Kaohsiung J Med Sci. 1996 Feb;12(2):83-7.
Diminished actin response to N-formyl-methionyl-leucyl-phenylalanine (FMLP) has been used to explain the impaired chemotaxis of neonatal neutrophils, but the effect of labor on this response has not been evaluated before. Therefore, we tested the hypothesis that labor stress may have an effect on actin response of cord blood neutrophils to FMLP. This response is compared by phallacidin stain of neutrophil F-actin and flow cytometry analysis among 3 groups of healthy neonates: group I, 16 vaginally delivered neonates; group II, 16 neonates delivered by elective cesarean section without labor; and group III, 16 neonates delivered by cesarean section after labor. In the group III, 10 infants were delivered by an emergency cesarean section because of fetal heart rate deceleration indicating fetal distress (one minute Apgar score < 7 in 5 cases [50%]), and the other 6 infants were delivered because of failure of progression of labor (one minute Apgar score < 7 in 2 cases [33.3%]). Stimulated relative F-actin content of neutrophils in group III was increased compared with groups I and II. There was no such difference between neutrophils from infants of groups I and II. The results of this study indicate that fetal distress may enhance neutrophil actin response to FMLP, while labor or mode of delivery might not have such effect.
肌动蛋白对N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（FMLP）的反应减弱已被用来解释新生儿中性粒细胞趋化性受损的原因，但此前尚未评估分娩对这种反应的影响。因此，我们检验了这样一个假设，即分娩应激可能会影响脐血中性粒细胞对FMLP的肌动蛋白反应。通过用鬼笔环肽对中性粒细胞F-肌动蛋白进行染色，并采用流式细胞术分析，对3组健康新生儿的这种反应进行了比较：第一组，16名经阴道分娩的新生儿；第二组，16名择期剖宫产且未经历分娩的新生儿；第三组，16名经历分娩后剖宫产的新生儿。在第三组中，10名婴儿因胎儿心率减速提示胎儿窘迫而行急诊剖宫产（5例[50%]一分钟阿氏评分<7），另外6名婴儿因产程进展不佳而行剖宫产（2例[33.3%]一分钟阿氏评分<7）。与第一组和第二组相比，第三组中受刺激的中性粒细胞相对F-肌动蛋白含量增加。第一组和第二组婴儿的中性粒细胞之间没有这种差异。本研究结果表明，胎儿窘迫可能会增强中性粒细胞对FMLP的肌动蛋白反应，而分娩或分娩方式可能没有这种作用。
