Holt P R, Mokuolu A O, Distler P, Liu T, Reddy B S
Gastrointestinal Division, St. Luke's-Roosevelt Hospital Center, College of Physicians and Surgeons, Columbia University, New York, NY 10025, USA.
Nutr Cancer. 1996;25(2):129-35. doi: 10.1080/01635589609514435.
Carcinogen induction of neoplasms in rodent colon has been used as a model for human colon cancer development and for evaluating chemopreventive regimens. We studied the regional distribution of small and large intestinal tumors in 229 rats given azoxymethane (AOM) once weekly for two weeks (15 mg/kg sc). The AOM regimen induced 63% more tumors in distal (DC) than in proximal colon (PC), although tumor volume was greater in PC. A high-fat (23% corn oil) diet increased tumors in PC and DC (p < 0.01). Caloric restriction of 10-30% of the ad libitum diet progressively reduced DC tumor formation but did not alter PC tumors. Tumor volume was unaffected by either regimen. Small intestinal tumors were concentrated in the proximal 15 cm of the intestine and were unaffected by dietary manipulation. This AOM model of colon tumor formation approximates human colon cancer distribution and is an appropriate model for rodent chemopreventive studies.
致癌物诱导啮齿动物结肠肿瘤已被用作人类结肠癌发展的模型以及评估化学预防方案的模型。我们研究了229只大鼠的小肠和大肠肿瘤的区域分布，这些大鼠每周皮下注射一次偶氮甲烷（AOM），共注射两周（15mg/kg）。AOM方案诱导远端结肠（DC）的肿瘤比近端结肠（PC）多63%，尽管PC中的肿瘤体积更大。高脂肪（23%玉米油）饮食增加了PC和DC中的肿瘤（p<0.01）。将自由采食饮食热量限制10%-30%可逐渐减少DC肿瘤形成，但不改变PC肿瘤。两种方案均未影响肿瘤体积。小肠肿瘤集中在小肠近端15cm处，不受饮食操作的影响。这种AOM诱导的结肠肿瘤形成模型近似于人类结肠癌分布，是啮齿动物化学预防研究的合适模型。
